Scientists cut HIV out of human genome

Reuters / Suzanne Plunkett

Scientists from Temple University School of Medicine in Philadelphia have pioneered a technique which uses a targeting strand of RNA (called guide RNA, or gRNA) that locates the virus. A “DNA snipping” enzyme (called a nuclease) then removes it from the infected cell. The cell, now free of the HIV virus, then repairs itself.

Cells armed with the nuclease-RNA combination proved impervious to HIV infection, the research states.

This is an important step on the path toward a permanent cure for AIDS. It’s an exciting discovery, but it’s not yet ready to go into the clinic. It’s a proof of concept that we’re moving in the right direction,” said Dr. Khalili, director of the Comprehensive NeuroAIDS Center at Temple and lead author of the study.

The editing process was successful in several cell types that can harbor HIV-1, including microglia and macrophages, as well as T-lymphocytes. “T-cells and monocytic cells are the main cell types infected by HIV-1, so they are the most important targets for this technology,” Khalili said.

The research was published by the Proceedings of the National Academy of Sciences on Monday.

The HIV virus operates by permanently inserting its genome into a patient’s DNA, forcing them into a lifelong drugs regimen of antiretroviral therapy (HAART) to control the disease.

But, although the HAART treatment allows people to live a relatively normal life, there are growing problems of HIV drug resistance and the virus becomes active again if treatment is ever stopped.

Scientists have been struggling to find a cure for HIV for more than three decades, but the virus’ ability to “replicate unrelentingly despite everything the immune system can throw at it,” have made a cure an elusive goal, according to virologist Ron Desrosiers.

Since HIV-1 is never cleared by the immune system, removal of the virus is required in order to cure the disease,” said Khalili.

But serious problems remain with the new technique. Scientists have still not found a way to deliver the DNA snipping enzymes to every cell, and there are concerns that the treatment could lead to unexpected side effects like cell mutations.

There is also a worry that the genetic diversity of the HIV virus – it is prone to mutations – may mean that treatment has to be tailored towards each strand of the virus.

So far, researchers have only targeted the virus in laboratory test tubes. But Dr. Khalili is optimistic that his technique is the best hope for a cure.

We are working on a number of strategies so we can take the construct into preclinical studies. We want to eradicate every single copy of HIV-1 from the patient. That will cure AIDS. I think this technology is the way we can do it,” he said.

There are around 35 million people worldwide infected with HIV; 1.6 million died from AIDS related illnesses in 2012 – most of them in the third world, where treatment is not as advanced or available, according to the World Health Organization.

US intelligence: No link to Russia in Malaysia plane downing

A part of the wreckage is seen at the crash site of the Malaysia Airlines Flight MH17 near the village of Hrabove (Grabovo), in the Donetsk region July 21, 2014.(Reuters / Maxim Zmeyev)

Officials believe that the passenger aircraft was intercepted by an SA-11 surface-to-air missile, which was fired by Ukrainian militia members. One official said the likeliest explanation was the aircraft was shot down in error, an assertion that seem to be bolstered by the previous downing of 12 Ukrainian military aircraft by militants in the region.

Intelligence suggests that, although the US maintains that Russia “created the conditions” that led to the incident, officials were not aware of the presence of any Russians during the missile launch, and would not confirm that the missile crew was trained in Russia.

The crash of the airliner last week with 298 passengers aboard has only exacerbated the row between the US, European powers and Russia over the developing situation in eastern Ukraine.

The aircraft’s black boxes were turned over to Malaysian authorities late Monday night by Ukrainian militia. Access to the debris field, which is located within the restive Donetsk People’s Republic, has been limited and rumors have run rampant regarding the conduct of militia in the area.

One official said that, regarding as to who precisely fired the missile, “we don’t know a name, we don’t know a rank and we’re not even 100 percent sure of a nationality.” The official added that “there is not going to be a Perry Mason moment here,” referring to any likely absence of definitive conclusions.

Officials noted that their inquiry relied partly on social media postings, citing specifically video of a missile launcher purported to be a Buk system battery crossing into Russian territory, and appearing to be missing a missile. Following questions, intel officials admitted they had not verified the video’s origin or content.

The Tuesday briefing seemed to be a sharp departure from comments made by US President Barack Obama a day prior, who stated that the Malaysia Airlines aircraft had been “shot down over territory controlled by Russian-backed separatists” that had been both armed with anti-aircraft weapons and trained by Russia.

Former VP Candidate Asks: “Is Obama Waging Jihad Against America?”

Wayne Allyn Root says he has always taken President Barack Obama at his word when he says he’s an American-born Christian. Now that he’s had over five years to watch this president’s actions in office, he still takes him at his word–sort of. Root believes that “American-born Christian” doesn’t fully describe Barack Obama. “I’ve met


This is now the second occurrence of a 24 hour flight shut down in Israel. Just a little while ago the US shut down their embassy in Israel for 24 hours. Now they have completely shutdown flying. How many more incidences have to occur before they shut down flying all together? This is part of a plan to bring about Martial Law in the United States shutting down flying outside of the borders effectively locks down the fastest way to leave the USA. All of this is going to come to pass. Now the bigger issue, Israel. Gaza has been home to a lot of violence over the years, but now Israel is going down in flames. This is the beginning of world war 3 because all will be divided against each other.

Another major issue, MH17. The second false flag from Malaysia to have a major death toll this year. In the beginning of this year a plane mysteriously disappeared, which in the modern age is completely impossible. Now just last week a Malaysian jet was shot out of the sky over Ukraine, another devastated war zone! This incident is going to be used to start a major war around the world! Both Russia and China say that is Ukraine and NATO, but USA and NATO say it was Russia. This very incident is already having ripple effects because the EU is now going to impose tougher sanctions on Russia beginning Thursday. Even though the investigation is still underway. Not much time left as the world burns faster and faster each day, violence has consumed whole countries like Iraq, and the Christian genocide that is going on there no one will speak about. The ISIS militants have completely destroyed the region including thousands of years worth of traditions. As they rape, murder, eat, mutilate Christians; Obama and the White House says absolutely nothing about the current crisis in the region. In fact he is so worried about Iraq and Syria that he is going to go party it up at the end of this month as he takes yet another vacation!

The end of America is nigh, with the border being completely overrun by drug cartels, Mexican military, Foreign soldiers, and then a bombshell report comes out. Border patrol says the border is run not by the US but by the UN! Is this why we see all the UN trucks heading that way? You better not believe that, the UN is coming to disarm Americans. However some good news has come out of this and that is governor Rick Perry has decided to send in the National Guard, roughly 1,000 troops will be deployed onto the border, that is good news. However the timing is all to familiar, most likely the USA is going to be going to war with Mexico and what is in Mexico is not just Mexican military, but military from around the world! This country is going to burn, and when the mainstream asks why nothing was done just look at Obama as he throws back another 6 pack and laughs off the demise of America!

This One Picture Explains Perfectly Why Israel is Right to Fight Back [PICTURE]

They say a picture is worth a thousand words. That’s certainly what the Israel Defense Forces hope is true as they attempt to help others understand their decision to engage in military action in the Gaza Strip. The IDF recently posted an obviously doctored image of rockets flying over the British Parliament, which has also

Bus carrying 42 school children crashes and overturns in Norfolk

Reuters / Regis Duvignau

Although there were no casualties, the driver sustained back injuries and had to be cut out of the vehicle. He was subsequently taken to Kings Lynn Hospital.

Another child was also transferred to hospital as a result of an ongoing medical condition, according to East England Ambulance Service.

Emergency services were alerted of the accident at 11 a.m. local time, following reports of a coach crashing at Hilgay in Norfolk. The passengers were on a day trip to the Norfolk coast.

The children, all of whom were protected by seat belts, are pupils at William Westley Primary School in Whittlesford, Cambridgeshire.

All passengers except for the bus driver were able to leave the vehicle immediately, according to local police.

In the aftermath of the incident, a number of passengers were in shock and received treatment at the scene. The A10 was closed while the cause of the crash was investigated. No other vehicles were involved.

A spokesman for Cambridgeshire County Council told the Wisbech Standard: “The bus which ended up in the ditch was one of three traveling in convoy so the children simply got on to the other two buses and are being taken back to the school. All of them are unhurt.”

“A good outcome to what could have been a catastrophe”: Relief as kids escape bus crash unhurt

— ITV News Anglia (@itvanglia) July 22, 2014

Three separate ambulances, an air ambulance, and an array of fire engines were deployed to deal with the incident, local paramedics said.

Terry Hicks arrived at the scene on behalf of the East of England Ambulance Service.

“Everyone was very calm and the children were a credit to both their parents and their teachers – and all of the emergency services worked well together at the scene to deal with the incident as quickly and smoothly as possible,” he said.

Heartrending Gaza: Reporter breaks into tears live on air (VIDEO)

Screenshot from YouTube user Steven Mirrow

The correspondent, who had just returned from Gaza’s Shejaia neighborhood – which came under heavy Israeli bombardment on Sunday, leaving dozens dead – began speaking about what he and his TV crew witnessed. He stated that his team saw a lot of bodies, adding that the crew was in a state of shock.

However, as he was describing the “laying bodies,” he broke into tears. He tried to compose himself and continue, but subsequently walked off screen.

Tuesday saw the Gaza conflict move into its third week. According to the Gaza Health Ministry, the number of Palestinians killed has risen to 609 – the vast majority of whom are civilians.

Al Jazeera has claimed that its offices in Gaza were targeted by the Israel Defense Forces. One of the channel’s correspondents reported that the building came under fire on Tuesday. According to staff members, the 11th floor of the bureau was hit by two Israeli bullets as a crew was preparing to broadcast live from the balcony, the Guardian reported.

However, a spokesman for the Israel Defense Forces said that no warning shots were fired, the Jerusalem Post reported. He could not confirm or deny whether indirect damage to the building occurred during the targeting of nearby military targets.

Al Jazeera Gaza Bureau chief shows high caliber round he says was one of at least two shot into the office by Israel

— Tamer El-Ghobashy (@TamerELG) July 22, 2014

Sarah Palin: Join the Movement to Impeach Barack Obama [WATCH SPEECH]

Calls to impeach Barack Obama have increased weekly as news develops regarding the immigration crisis that he precipitated with his signature Deferred Action for Childhood Arrivals (DACA) policy. In direct response to this policy, tens of thousands of illegal immigrants, mostly from Central America, have entered the country, confident that they will be allowed to